Corrections

Teks daripada monica_tei - English

  • Basic research project

    • The advance in regenerative medicine, especially in the field of cardiology, is due, among other factors, to obtaining induced pluripotency stem cells (iPS), which makes it possible to study diseases such as anthracycline-induced cardiotoxicity (CIA ).
    • A class of more effective chemotherapeutics used as a therapeutic measure to treat neoplasms are anthracyclines.
    • Its antineoplastic effects are considered to involve DNA strand intercalation, stabilization of the topoisomerase IIα cleavage complex and imbalance in the redox system, generating an accumulation of toxic and reactive oxygen radicals, inducing apoptosis.
    • Despite the benefit generated in the treatment of cancer, its clinical use is hampered by the risk of cardiotoxicity and it is still not known what predisposes the individual to develop such a side effect.
    • Due to cardiotoxic side effects, there is considerable effort to develop cardioprotective drugs, but so far dexrazoxane (DXZ) is the only drug approved for the prevention of ASD.
    • Its probable cardioprotective effect is related to the removal of intracellular iron, preventing the production of the anthracycline-iron complex and subsequent production of toxic radicals, in addition to antagonizing the association of anthracyclines with topoisomerase IIβ, an isoform present in non-proliferating cells, such as cardiomyocytes.
    • It is speculated that, in this way, this cardioprotectant reduces the damage induced by anthracyclines to the DNA of cardiomyocytes.
    • Thus, the use of patient-specific cardiomyocytes differentiated from iPS (iPS-CM) represents a promising platform to perform pharmacological tests and study the mechanisms of cardiotoxicity induction.
    • The protocols that most efficiently mimic cardiomyogenesis use a base medium composed of RPMI 1640 plus B27, a supplement containing 21 components, originally developed for the culture of hippocampal neurons and recently adapted for differentiation into iPS-CM.
    • As most of the B27 component molecules are antioxidants, it is possible that their addition causes a cardioprotective effect on the iPS-CM generated in vitro, possibly altering their maturation and the observed results.
    • Thus, the objective of this work is to establish a protocol that presents high efficiency of cardiac differentiation (CO), but that does not make use of the supplement B27.
    • In this work, we used two strains of iPS: one obtained from a patient treated with cumulative doses of anthracyclines (240 mg/m2 doxorubicin and 300 mg/m2 epirubicin) and who developed heart failure (ejection fraction = 32.9%) after 7 years of treatment, and another from a healthy donor (control).
    • Patient-specific iPS underwent CD using two different protocols: P1 – using B27 (RPMI 1640 and supplement B27 1X serum free or B27 minus insulin), and P2 – CDM3 (RPMI 1640, 213 μg/mL L-ascorbic acid 2 -phosphate, 500 μg/mL bovine serum albumin).
  • Both temporarily modulate Wnt signaling, activating it with CHIR99021 for differentiation into mesoderm, and inhibiting it with XAV939 (P1)/Wnt-C59 (P2) for cardiac specification.
    • Starting from the eighth day, the spontaneous contractility of the iPS-CM was evaluated by optical phase contrast microscopy.
    • After thirty days, we evaluated by flow cytometry the efficiency of the two protocols by cardiac troponin T labeling and obtained efficiency >90%.
    • With this, we were able to obtain iPS-CM through an efficient and reproducible protocol, using a medium without B27.

MOHON BANTU UNTUK BETULKAN SETIAP AYAT! - English

  • Title
  • Ayat 1
    • The advance in regenerative medicine, especially in the field of cardiology, is due, among other factors, to obtaining induced pluripotency stem cells (iPS), which makes it possible to study diseases such as anthracycline-induced cardiotoxicity (CIA ).
      100% GOOD (1 votes)
    • The advance in regenerative medicine, especially in the field of cardiology, is due, among other factors, to obtaining induced pluripotency stem cells (iIPS), which makes it possible to study diseases such as anthracycline-induced cardiotoxicity (CIA ).

      NOTE: Good work. About ”anthracycline-induced cardiotoxicity ” -- sure it cannot be abbreviated as AIC, rather than CIA? CIA is, well, kinda already taken, you know?
    • TAMBAH PEMBETULAN YANG BARU! - Ayat 1TAMBAH PEMBETULAN YANG BARU! - Ayat 1
  • Ayat 2
  • Ayat 3
    • Its antineoplastic effects are considered to involve DNA strand intercalation, stabilization of the topoisomerase IIα cleavage complex and imbalance in the redox system, generating an accumulation of toxic and reactive oxygen radicals, inducing apoptosis.
      Undi sekarang!
    • Its antineoplastic effects are considered to involve DNA strand intercalation, stabilization of the topoisomerase IIα cleavage complex and imbalance in the redox system, generating an accumulation of toxic and reactive oxygen radicals, inducing apoptosis.
    • Its antineoplasticTheir effects are considered to involve DNA strand intercalation, stabilization of the topoisomerase IIα cleavage complex and imbalance in the redox system, generating an accumulation of toxic and reactive oxygen radicals, inducing apoptosis.
    • TAMBAH PEMBETULAN YANG BARU! - Ayat 3TAMBAH PEMBETULAN YANG BARU! - Ayat 3
  • Ayat 4
    • Despite the benefit generated in the treatment of cancer, its clinical use is hampered by the risk of cardiotoxicity and it is still not known what predisposes the individual to develop such a side effect.
      Undi sekarang!
    • Despite theits benefit generateds in the treatment of cancer, its clinical use is hampered by the risk of cardiotoxicity and it is still not knownstill little in known regarding what predisposes the individual to develop such a side effect.
    • Despite the benefit generatedir derived benefits in the treatment of cancer, itsthe clinical use of anthracyclines is hampered by the risk of cardiotoxicity and it is still not knownwith nothing known regarding what predisposes thean individual to develop such a side effect.
    • TAMBAH PEMBETULAN YANG BARU! - Ayat 4TAMBAH PEMBETULAN YANG BARU! - Ayat 4
  • Ayat 5
    • Due to cardiotoxic side effects, there is considerable effort to develop cardioprotective drugs, but so far dexrazoxane (DXZ) is the only drug approved for the prevention of ASD.
      100% GOOD (1 votes)
    • Due to cardiotoxic side effects, there is considerable effort to develop cardioprotective drugs, but so far dexrazoxane (DXZ) is the only drug approved for the prevention of ASD.

      NOTE: What does ASD stand for?
    • Due to their cardiotoxic side effects, there is considerable effort to develop cardioprotective drugs, but so far; previously dexrazoxane (DXZ) ishas been the only drug which has undergone regulatory approvedal for the prevention of (express explicitly what ASD is, unless it is a common acronym for the technical audience of your paper) ASD.
    • TAMBAH PEMBETULAN YANG BARU! - Ayat 5TAMBAH PEMBETULAN YANG BARU! - Ayat 5
  • Ayat 6
    • Its probable cardioprotective effect is related to the removal of intracellular iron, preventing the production of the anthracycline-iron complex and subsequent production of toxic radicals, in addition to antagonizing the association of anthracyclines with topoisomerase IIβ, an isoform present in non-proliferating cells, such as cardiomyocytes.
      100% GOOD (1 votes)
    • ItsThe probable cardioprotective effect of DXZ is related to the removal of intracellular iron, preventing the production of thean anthracycline-iron complex and subsequent production of toxic radicals, in addition to antagonizing the association of anthracyclines with topoisomerase IIβ, an isoform present in non-proliferating cells, such as cardiomyocytes.
    • TAMBAH PEMBETULAN YANG BARU! - Ayat 6TAMBAH PEMBETULAN YANG BARU! - Ayat 6
  • Ayat 7
    • It is speculated that, in this way, this cardioprotectant reduces the damage induced by anthracyclines to the DNA of cardiomyocytes.
      Undi sekarang!
    • It is speculated that, in this way, this cardioprotectant reduces the damage induced by anthracyclines to the DNA of cardiomyocytes.

      NOTE: Wording is a bit rough -- clumsy more than anything else -- but it's acceptable.
    • It is speculated that, in this wayvia such a process, this cardioprotectant reduces the damage induced by anthracyclines to the DNA of cardiomyocytes.
    • TAMBAH PEMBETULAN YANG BARU! - Ayat 7TAMBAH PEMBETULAN YANG BARU! - Ayat 7
  • Ayat 8
    • Thus, the use of patient-specific cardiomyocytes differentiated from iPS (iPS-CM) represents a promising platform to perform pharmacological tests and study the mechanisms of cardiotoxicity induction.
      100% GOOD (1 votes)
    • Thus, the use of patient-specific cardiomyocytes differentiated from iPS (iPS-CM) represents a promising platform to perform pharmacological tests and study the mechanisms of cardiotoxicity induction.

      NOTE: so the abbreviateion is "iPS"and not "IPS" -- i.e., the letter "i" is not capitalized in the abbreviation?
    • Thus, the use of patient-specific cardiomyocytes differentiated from iPS (iPS-CM) represents a promising platform on which to perform pharmacological testsing and study the mechanisms of cardiotoxicity induction.
    • TAMBAH PEMBETULAN YANG BARU! - Ayat 8TAMBAH PEMBETULAN YANG BARU! - Ayat 8
  • Ayat 9
    • The protocols that most efficiently mimic cardiomyogenesis use a base medium composed of RPMI 1640 plus B27, a supplement containing 21 components, originally developed for the culture of hippocampal neurons and recently adapted for differentiation into iPS-CM.
      100% GOOD (1 votes)
    • The protocols that most efficiently mimicing cardiomyogenesis use a base medium composed of RPMI 1640 plus B27, a supplement containing 21 components, originally developed for the culture of hippocampal neurons and recently adapted for differentiation into iPS-CM.
    • TAMBAH PEMBETULAN YANG BARU! - Ayat 9TAMBAH PEMBETULAN YANG BARU! - Ayat 9
  • Ayat 10
    • As most of the B27 component molecules are antioxidants, it is possible that their addition causes a cardioprotective effect on the iPS-CM generated in vitro, possibly altering their maturation and the observed results.
      Undi sekarang!
    • As most of the B27 component molecules are antioxidants, it is possible that their addition causes a cardioprotective effect on the iPS-CM generated in vitro, possibly altering their maturation and the observed results.
    • As most of the B27 component molecules are antioxidants, it is possible that their addition causes a cardioprotective effect on the iPS-CM generated in vitro, possibly altering their maturation and the observed results.
    • TAMBAH PEMBETULAN YANG BARU! - Ayat 10TAMBAH PEMBETULAN YANG BARU! - Ayat 10
  • Ayat 11
    • Thus, the objective of this work is to establish a protocol that presents high efficiency of cardiac differentiation (CO), but that does not make use of the supplement B27.
      Undi sekarang!
    • Thus, the objective of this work is to establish a protocol that presents highly efficiencyt protocol of cardiac differentiation (CO), but that does not make use of the supplement B27.

      NOTE: Should the abbreviation for "cardiac differentiation" be "CO" or "CD"?
    • Thus, the objective of this work is tohe establishment of a protocol that presents high efficiency of cardiac differentiation (CO), but that does not make use ofby which cardiac differentiation (CD) is highly efficient, but nevertheless does not utilise the supplement B27.
    • TAMBAH PEMBETULAN YANG BARU! - Ayat 11TAMBAH PEMBETULAN YANG BARU! - Ayat 11
  • Ayat 12
    • In this work, we used two strains of iPS: one obtained from a patient treated with cumulative doses of anthracyclines (240 mg/m2 doxorubicin and 300 mg/m2 epirubicin) and who developed heart failure (ejection fraction = 32.9%) after 7 years of treatment, and another from a healthy donor (control).
      Undi sekarang!
    • In this work, we used two strains of iPS: one obtained from a patient treated with cumulative doses of anthracyclines (240 mg/m2 doxorubicin and 300 mg/m2 epirubicin) and who developed heart failure (ejection fraction = 32.9%) after 7 years of treatment, and another obtained from a healthy donor (control).
    • In this work, we used two strains of iPS: one obtained from a patient treated with cumulative doses of anthracyclines (240 mg/m2 doxorubicin and 300 mg/m2 epirubicin) and whowho consequently developed heart failure (ejection fraction = 32.9%) after 7 years of treatment, and another from a healthy donor (- the control).
    • TAMBAH PEMBETULAN YANG BARU! - Ayat 12TAMBAH PEMBETULAN YANG BARU! - Ayat 12
  • Ayat 13
    • Patient-specific iPS underwent CD using two different protocols: P1 – using B27 (RPMI 1640 and supplement B27 1X serum free or B27 minus insulin), and P2 – CDM3 (RPMI 1640, 213 μg/mL L-ascorbic acid 2 -phosphate, 500 μg/mL bovine serum albumin).
      Undi sekarang!
    • Patient-specific iPS underwent CD utilising two different protocols: P1 – using B27 (RPMI 1640 and supplement B27 1X serum free or B27 minus insulin), and P2 – CDM3 (RPMI 1640, 213 μg/mL L-ascorbic acid 2 -phosphate, 500 μg/mL bovine serum albumin).
    • TAMBAH PEMBETULAN YANG BARU! - Ayat 13TAMBAH PEMBETULAN YANG BARU! - Ayat 13
  • Ayat 14
    • Both temporarily modulate Wnt signaling, activating it with CHIR99021 for differentiation into mesoderm, and inhibiting it with XAV939 (P1)/Wnt-C59 (P2) for cardiac specification.
      Undi sekarang!
    • TAMBAH PEMBETULAN YANG BARU! - Ayat 14TAMBAH PEMBETULAN YANG BARU! - Ayat 14
  • Ayat 15
    • Starting from the eighth day, the spontaneous contractility of the iPS-CM was evaluated by optical phase contrast microscopy.
      100% GOOD (1 votes)
    • Starting from the eighth dayFrom the eighth day (of what??? - not clear from your context here exactly what you mean!), the spontaneous contractility of the iPS-CM was evaluated by optical phase contrast microscopy.
    • TAMBAH PEMBETULAN YANG BARU! - Ayat 15TAMBAH PEMBETULAN YANG BARU! - Ayat 15
  • Ayat 16
    • After thirty days, we evaluated by flow cytometry the efficiency of the two protocols by cardiac troponin T labeling and obtained efficiency >90%.
      Undi sekarang!
    • After thirty days, we evaluated byused flow cytometry to evaluate the efficiency of the two protocols by cardiac troponin T labeling and obtained efficiency >90%.
    • After thirty days, we evaluated byvia flow cytometry the efficiency of the two protocols by cardiac troponin T labeling and obtained an efficiency >90%.
    • TAMBAH PEMBETULAN YANG BARU! - Ayat 16TAMBAH PEMBETULAN YANG BARU! - Ayat 16
  • Ayat 17
    • With this, we were able to obtain iPS-CM through an efficient and reproducible protocol, using a medium without B27.
      Undi sekarang!
    • With this,Thus we were able to obtain iPS-CM through an efficient and reproducible protocol, using a medium without B27. ¶
    • With this, we were able to obtain iPS-CMUsing such a methodology, iPS-CM is obtained through an efficient and reproducible protocol, utilising a medium without B27. ¶
    • TAMBAH PEMBETULAN YANG BARU! - Ayat 17TAMBAH PEMBETULAN YANG BARU! - Ayat 17