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Tekst van Heresh - English

    • may correct grammatically

    • The high therapeutic potential of mesenchymal stem cells (MSCs) has significantly increased the interest in these cells in a wide range of medicals and veterinary.
  • MSCs are used to treat musculoskeletal disorders.
  • The differentiation of these cells into musculoskeletal tissue is regulated by the TGFβ family.
    • Various factors such as age, sex, species, source of isolation affect this distinction.
  • In this study, the expression of the TGFβ3 (TGFβR3) receptor as a factor involved in the musculoskeletal differentiation of mesenchymal stem cells derived from adipose tissue of different horses was investigated.
    • First, the frozen cells stored in the nitrogen tank were thawed and cultured to passage six with standard culture medium DMEM, FBS, and antibiotics. then Cells were collected for RNA extraction.
  • After extraction and qualitative and quantitative determination of the samples extracted by the nanodrop, RNA was converted to cDNA.
  • The qPCR reaction was performed for TGFβR3 genes as the target gene and GAPDH as the reference gene.
    • The data obtained from real-time were analyzed and the real-time product was run on agarose gel, where the single bands resulting from the amplification of these genes were seen.
    • The results showed that adipose tissue-derived mesenchymal stem cells of three different horses did not differ significantly in the relative expression of the TGFβR3 gene .In other words, the expression of this gene is not affected by the characteristics of the donor.

HELP ELKE ZIN TE CORRIGEREN, AUB! - English

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    • The high therapeutic potential of mesenchymal stem cells (MSCs) has significantly increased the interest in these cells in a wide range of medicals and veterinary.
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    • The high therapeutic potential of mesenchymal stem cells (MSCs) has significantly increased the interest in these cells in a wide range of medicals and veterinary applications.
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    • In this study, the expression of the TGFβ3 (TGFβR3) receptor as a factor involved in the musculoskeletal differentiation of mesenchymal stem cells derived from adipose tissue of different horses was investigated.
      Stem nu!
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  • Zin 6
    • First, the frozen cells stored in the nitrogen tank were thawed and cultured to passage six with standard culture medium DMEM, FBS, and antibiotics. then Cells were collected for RNA extraction.
      Stem nu!
    • First, the frozen cells stored in the nitrogen tank were thawed and cultured to passage six with standard culture medium DMEM, FBS, and antibiotics. then Ccells were collected for RNA extraction.
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    • First, the frozen cells stored in the nitrogen tank were thawed and cultured to passage six with standard culture medium DMEM, FBS, and antibiotics., then Ccells were collected for RNA extraction.
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    • The data obtained from real-time were analyzed and the real-time product was run on agarose gel, where the single bands resulting from the amplification of these genes were seen.
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    • ThRealk-time data were obtained from real-time werethen analyzed and the real-time product was run on agarose gel, where the single bands resulting from the amplification of these genes were seenobserved.
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  • Zin 10
    • The results showed that adipose tissue-derived mesenchymal stem cells of three different horses did not differ significantly in the relative expression of the TGFβR3 gene .In other words, the expression of this gene is not affected by the characteristics of the donor.
      Stem nu!
    • The results showed that adipose tissue-derived mesenchymal stem cells of three different horses did not differ significantly in the relative expression of the TGFβR3 gene .In other words, i.e., the expression of this gene is not affected by the characteristics of the donor.
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    • VOEG een NIEUWE CORRECTIE TOE! - Zin 10VOEG een NIEUWE CORRECTIE TOE! - Zin 10