Corrections

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Tekstong galing sa Heresh - English

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    • The high therapeutic potential of mesenchymal stem cells (MSCs) has significantly increased the interest in these cells in a wide range of medicals and veterinary.
  • MSCs are used to treat musculoskeletal disorders.
  • The differentiation of these cells into musculoskeletal tissue is regulated by the TGFβ family.
    • Various factors such as age, sex, species, source of isolation affect this distinction.
  • In this study, the expression of the TGFβ3 (TGFβR3) receptor as a factor involved in the musculoskeletal differentiation of mesenchymal stem cells derived from adipose tissue of different horses was investigated.
    • First, the frozen cells stored in the nitrogen tank were thawed and cultured to passage six with standard culture medium DMEM, FBS, and antibiotics. then Cells were collected for RNA extraction.
  • After extraction and qualitative and quantitative determination of the samples extracted by the nanodrop, RNA was converted to cDNA.
  • The qPCR reaction was performed for TGFβR3 genes as the target gene and GAPDH as the reference gene.
    • The data obtained from real-time were analyzed and the real-time product was run on agarose gel, where the single bands resulting from the amplification of these genes were seen.
  • The results showed that adipose tissue-derived mesenchymal stem cells of three different horses did not differ significantly in the relative expression of the TGFβR3 gene (P

Pakiusap, tumulong sa pagtatama sa mga pangungusap! - English