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    • The high therapeutic potential of mesenchymal stem cells (MSCs) has significantly increased the interest in these cells in a wide range of medicals and veterinary.
  • MSCs are used to treat musculoskeletal disorders.
  • The differentiation of these cells into musculoskeletal tissue is regulated by the TGFβ family.
    • Various factors such as age, sex, species, source of isolation affect this distinction.
  • In this study, the expression of the TGFβ3 (TGFβR3) receptor as a factor involved in the musculoskeletal differentiation of mesenchymal stem cells derived from adipose tissue of different horses was investigated.
    • First, the frozen cells stored in the nitrogen tank were thawed and cultured to passage six with standard culture medium DMEM, FBS, and antibiotics. then Cells were collected for RNA extraction.
  • After extraction and qualitative and quantitative determination of the samples extracted by the nanodrop, RNA was converted to cDNA.
  • The qPCR reaction was performed for TGFβR3 genes as the target gene and GAPDH as the reference gene.
    • The data obtained from real-time were analyzed and the real-time product was run on agarose gel, where the single bands resulting from the amplification of these genes were seen.
  • The results showed that adipose tissue-derived mesenchymal stem cells of three different horses did not differ significantly in the relative expression of the TGFβR3 gene (P

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    • The high therapeutic potential of mesenchymal stem cells (MSCs) has significantly increased the interest in these cells in a wide range of medicals and veterinary.
      0% GOOD (1 votes)
    • The high therapeutic potential of mesenchymal stem cells (MSCs) has significantly increased the interest in these cells in a wide range of medicals and veterinary fields.
      AlexweissAlexweiss profile picture Kommentarer
    • Tilføj en ny rettelse! - Sætning 1Tilføj en ny rettelse! - Sætning 1
  • Sætning 2
  • Sætning 3
  • Sætning 4
  • Sætning 5
    • In this study, the expression of the TGFβ3 (TGFβR3) receptor as a factor involved in the musculoskeletal differentiation of mesenchymal stem cells derived from adipose tissue of different horses was investigated.
      Stem nu!
    • Tilføj en ny rettelse! - Sætning 5Tilføj en ny rettelse! - Sætning 5
  • Sætning 6
    • First, the frozen cells stored in the nitrogen tank were thawed and cultured to passage six with standard culture medium DMEM, FBS, and antibiotics. then Cells were collected for RNA extraction.
      Stem nu!
    • First, the frozen cells stored in the nitrogen tank were thawed and cultured to passage six with standard culture medium (DMEM, FBS, and antibiotics). tThen Ccells were collected for RNA extraction.
      AlexweissAlexweiss profile picture Kommentarer
    • Tilføj en ny rettelse! - Sætning 6Tilføj en ny rettelse! - Sætning 6
  • Sætning 7
  • Sætning 8
  • Sætning 9
    • The data obtained from real-time were analyzed and the real-time product was run on agarose gel, where the single bands resulting from the amplification of these genes were seen.
      Stem nu!
    • The data obtained fromin real-time were analyzed, and the real-time product was run on agarose gel, where the single bands resulting from the amplification of these genes were seen.
      AlexweissAlexweiss profile picture Kommentarer
    • Tilføj en ny rettelse! - Sætning 9Tilføj en ny rettelse! - Sætning 9
  • Sætning 10
    • The results showed that adipose tissue-derived mesenchymal stem cells of three different horses did not differ significantly in the relative expression of the TGFβR3 gene (P
      Stem nu!
    • Tilføj en ny rettelse! - Sætning 10Tilføj en ny rettelse! - Sætning 10